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For each semen sample we determine the semen volume, sperm count (concentration), and percent motility (number of sperm that swim). We use a special solution (cryoprotectant) to protect the sperm from freezing conditions and place it into distinctive containers (cryovials) for freezing. Depending on the size and the quality of the sample, we will use one or more vials per sample. Each vial is labeled with your name, the freeze date and a unique vial number. The vials are slowly cooled, then frozen and stored in our liquid nitrogen tanks.